Journal: Journal of Thoracic Disease

Article Title: First-line treatment selection with organoids of an EGFR m + TP53 m stage IA1 patient with early metastatic recurrence after radical surgery and follow-up

doi: 10.21037/jtd-20-1882

Figure Lengend Snippet: Organoid culture additives

Article Snippet: Data analyses were performed using GraphPad Prism 7.0 software, and the values of IC50 were calculated by applying nonlinear regression (curve fit). table ft1 table-wrap mode="anchored" t5 caption a7 Additive Supplier Catalogue # Concentration N-Acetylcysteine Sigma A9165 1.25 mM EGF PeproTech AF-100-15 50 ng/mL FGF-10 PeproTech 100-26 20 ng/mL FGF-basic PeproTech 100-10B 1 ng/mL Y-27632 Selleck S1049 10 μM A-83-01 Sigma SML0788 500 nM SB202190 Selleck S1077 10 μM Nicotinamide Sigma 72340 10 mM PGE2 Tocris 2296 1 ìM Noggin (conditional medium) In-house – 50 mL R-Spondin (conditional medium) In-house – 50 mL HEPES Sigma H6147 10 mM GlutaMAX Gibco 35050061 1× B27 Gibco 17504044 1× N2 Gibco 17502048 1× Open in a separate window R-Spondin 1 and Noggin conditioned media were prepared in house from the culture media of Rspo1 cell and the noggin cDNA transfected HEK-293 cell following the methods described before ( 36 , 37 ). caption a8 Organoid culture additives

Techniques: Concentration Assay

Journal: Journal of Thoracic Disease

Article Title: First-line treatment selection with organoids of an EGFR m + TP53 m stage IA1 patient with early metastatic recurrence after radical surgery and follow-up

doi: 10.21037/jtd-20-1882

Figure Lengend Snippet: Organoid culture additives

Article Snippet: Data analyses were performed using GraphPad Prism 7.0 software, and the values of IC50 were calculated by applying nonlinear regression (curve fit). table ft1 table-wrap mode="anchored" t5 caption a7 Additive Supplier Catalogue # Concentration N-Acetylcysteine Sigma A9165 1.25 mM EGF PeproTech AF-100-15 50 ng/mL FGF-10 PeproTech 100-26 20 ng/mL FGF-basic PeproTech 100-10B 1 ng/mL Y-27632 Selleck S1049 10 μM A-83-01 Sigma SML0788 500 nM SB202190 Selleck S1077 10 μM Nicotinamide Sigma 72340 10 mM PGE2 Tocris 2296 1 ìM Noggin (conditional medium) In-house – 50 mL R-Spondin (conditional medium) In-house – 50 mL HEPES Sigma H6147 10 mM GlutaMAX Gibco 35050061 1× B27 Gibco 17504044 1× N2 Gibco 17502048 1× Open in a separate window R-Spondin 1 and Noggin conditioned media were prepared in house from the culture media of Rspo1 cell and the noggin cDNA transfected HEK-293 cell following the methods described before ( 36 , 37 ). caption a8 Organoid culture additives

Techniques: Concentration Assay